human colon mucosal epithelial cell line ncm460  (ATCC)

Journal: Journal of Inflammation Research

Article Title: Supplementation of Glucosamine Selenium Ameliorates DSS-Induced Chronic Colitis in Mice via Affecting Gut Microbiota, Inhibiting Pyroptosis and Inactivating Chemokine Signaling Pathway

doi: 10.2147/JIR.S486751

Figure Lengend Snippet: GASe-DS alleviates DSS-induced cell apoptosis and inflammatory response via inactivating chemokine signaling pathway. ( A ) The effects of series of GASe-DS content on cell viability. ( B ) 1% GASe-DS and reparixin increase cell viability, while ATI-2341 TFA decreases cell viability of DSS-treated NCM460 cells. ( C ) Flow cytometry shows that GASe-DS and reparixin treatment decrease cell apoptosis, while ATI-2341 TFA increases cell apoptosis. ( D ) Administration of GASe-DS and reparixin decrease the levels of IL-12, IL-6 and TNF-α. ATI-2341 TFA promotes the expression of IL-10, IL-6 and TNF-α. ( E ) Western blot analysis reveals that administration of GASe-DS and reparixin promote the expression of ZO-1, Occludin and claudin-1, and ATI-2341 TFA downregulates ZO-1, Occludin and claudin-1 in NCM460 cells. ( F ) Administration of GASe-DS and reparixin downregulate the expression of CXCL1 and CXCL2, which is reversed by ATI-2341 TFA in NCM460 cells. *** p < 0.001, compared with the control group. ### p < 0.001, compared with the DSS group. & p < 0.05, && p < 0.01, and &&& p < 0.001, compared with the DSS + GASe-DS group.

Article Snippet: NCM460, normal human colon mucosal epithelial cells, were obtained from icell Bioscience Inc. (Shanghai, China) and cultured in DMEM medium (HyClone, USA) containing 10% FBS (Biosera, France), 1% streptomycin-penicillin (Beyotime Institute of Biotechnology) at 37°C in an incubator with 95% humidity and 5% CO 2 .

Techniques: Flow Cytometry, Expressing, Western Blot, Control

Journal: BMC Cancer

Article Title: A novel machine learning-based immune prognostic signature for improving clinical outcomes and guiding therapy in colorectal cancer: an integrated bioinformatics and experimental study

doi: 10.1186/s12885-025-13437-0

Figure Lengend Snippet: Functional analysis of the representative gene APCDD1 in IRPS ( A ) Comparison of the APCDD1 expression level between CRC tissues and normal control tissues based on the GEPIA2 database; ( B ) The expression level of APCDD1 in CRC lines SW620, HT29 and SW480 was detected by qRT-PCR with the normal colon epithelial cell line NCM460 as a control; ( C ) The siRNA interference efficiency of APCDD1 in HT29 cell line was detected by qRT-PCR; ( D ) CCK-8 assays were used to detect the effect of APCDD1 knockdown on HT29 cell proliferation; ( E and F ) Transwell assays were used to detect the effect of APCDD1 knockdown on HT29 cell migration ( E ) and invasion ( F ); ( G ) Apoptosis assays were used to detect the effect of APCDD1 knockdown on apoptosis of HT29 cells. * P < 0.05; ** P < 0.01; *** P < 0.001

Article Snippet: The human CRC cell lines HT29, SW480 and SW620, as well as the human normal colon mucosal epithelial cell line NCM460, were purchased from KeyGEN BioTECH Corp., Ltd (China).

Techniques: Functional Assay, Comparison, Expressing, Control, Quantitative RT-PCR, CCK-8 Assay, Knockdown, Migration